We set a new standard in protein drinks.
We’re not your typical supplement brand. Instead of tweaking what’s already out there, we spent two years on research and development to bring a true innovation to the protein drink market.
But let’s start at the beginning...
PART 01
Protein
So, what is exactly a protein?
Protein is one of the body’s most important components. It helps build and repair muscles, skin, organs and other tissues, and it also plays a key role in making enzymes and hormones that keep your body working properly.
The building blocks of protein
Proteins are made up of amino acids, also known as the building blocks of protein. There are 20 different amino acids, each with its own distinct role in the body: one may trigger muscle protein synthesis — the key process for building new muscle tissue — while another supports energy production during exercise and helps regulate blood sugar.
Amino acids are grouped into two categories: essential and non-essential.
Your body cannot produce these amino acids on its own. You must obtain them from diet or supplements.
These are a smaller subset of essential amino acids, made up of three with a special branched shape. They all play an important role in muscle recovery.
Builds collagen, supports immune system
Detox function, produces other amino acids
Supports skin and gut health
Makes dopamine (mood + focus)
Makes serotonin (sleep + mood)
Makes histamine (immune + stomach acid)
Triggers muscle growth
Energy during exercise
Muscle repair & energy
Non-essential amino acids are produced naturally by the body and don’t need to be consumed through food.
Helps convert glucose into energy and supports muscle endurance
Helps convert glucose into energy and supports muscle endurance
Plays a role in energy production and hormone regulation
Supports detoxification and antioxidant production
Precursor to dopamine and thyroid hormones
Supports gut health and immune function
Acts as a key neurotransmitter for brain function
Contributes to collagen production and sleep quality
Essential for collagen formation and joint health
Improves blood flow and nitric oxide production
Supports brain function and cell membrane formation
Animal and plant based proteins
Protein comes from many sources – plant-based options like beans, nuts, and certain grains, or animal-based options like eggs, fish, meat, and dairy products such as milk and yogurt. The same distinction applies to protein supplements, which are also divided into plant- and animal-based types.
ANIMAL BASED PROTEIN
Animal proteins are considered “complete” proteins, meaning they naturally contain all the 9 essential amino acids in sufficient amounts.
- Whey protein
- Casein protein
- Collagen protein (bovine, chicken, marine)
Plant based protein
Plant proteins are vegan-friendly and easy to digest, but they do not naturally contain all essential amino acids and are lower in BCAAs.
- Pea protein
- Soy protein
- Rice protein
Whey protein as the gold standard
Of all protein types, whey protein is the most effective for muscle recovery and growth. This is because whey provides a complete amino acid profile, contains the highest amount of BCAAs and is particularly rich in leucine — the amino acid that acts as the key trigger for mTOR activation, the process that initiates muscle protein synthesis (MPS).
This is why whey remains the most trusted and widely used protein supplement worldwide.
Whey protein comes in 3 different forms:
Concentrate
Processing: Lightly filtered to remove some fat and lactose, keeping more of whey’s natural nutrients.
Protein content: ~70–80% protein by weight.
Key feature: Slightly slower absorption and higher in calories. Ideal for those looking to gain weight.
Isolate
Processing: Further filtered (cross-flow microfiltration or ion exchange) to remove almost all fat and lactose.
Protein content: ~90%+ protein by weight.
Key feature: Faster absorption, low in fat and lactose. It’s the most widely used form of whey protein worldwide.
Hydrolysate
Processing: Pre-digested through enzymatic hydrolysis, breaking protein chains into smaller peptides.
Protein content: ~90% protein by weight.
Key feature: Fastest absorption and less allergenic, but it has a slightly bitter taste and comes at a higher price.
How whey protein is made
The milks used for whey protein supplements is derived from cows.
The raw unpasteurized milk is collected and cooled to °4 C
The milk is transported in a refrigerated tank to a cheese making facility.
Milk is pasteurized at 72- 73 °C and recooled. This milk is 80% casein and 20% whey.
Milk undergoes cheese processing, and the liquid whey is drained off.
The liquid whey is collected and sent to be filtered to remove fat, lactose, minerals and water.
Whey protein is microfiltered to become whey protein concentrate (WPC) and ultrafiltered to make whey isolate (WPI).
The high protein liquid whey is spray-dried to become whey protein powder.
The protein is packaged into large bags and stored under optimal conditions.
The protein undergoes extensive testing to assess its purity and quality.
The protein is supplied to producers of protein powders or RTD drinks.
Finally, your whey protein product is ready to consume and enjoy.
Learn more about leucine
Your recovery starts with leucine.
You’ve probably never heard of leucine, MPS, or the leucine threshold. No worries — let’s start with some basics about protein first:
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1
Proteins are made from amino acids, which act like tiny building blocks— put them together, and you get a protein.
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2
There are 20 different amino acids, each with its own distinct role in the body: some help repair muscles, while others support energy production during exercise and help regulate blood sugar.
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3
Amino acids can be divided into two groups: non-essential amino acids, which the body produces on its own, and essential amino acids (EAAs), which must be obtained through the diet.
Essential amino acids
These 9 amino acids cannot be produced by your body and must come from food or supplements.
Amino acids: Lysine, Methionine, Threonine, Phenylalanine, Tryptophan, Histidine, Leucine*, Isoleucine*, Valine*
Non-essential amino acids
These 11 amino acids are produced naturally by the body and therefore are not required from diet.
Amino acids: Alanine, Glutamic, Glycine, Asparagine, Serine, Proline, Glutamine, Arginine, Glutamine, Cysteine, Tyrosine, Aspartic acid
* These are branched-chain amino acids (BCAAs), a special subset of EAAs that play a key role in muscle recovery and growth.
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4
Branched-Chain Amino Acids (BCAAs), are a group of 3 EAAs: leucine, isoleucine, and valine. They are called “branched-chain” because of their chemical structure, which looks like a branch.
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5
Whey protein is not a single protein: it is actually a blend of several proteins, known as protein fractions. Each fraction has a unique amino acid profile, which means that the proportion of individual amino acids differs between fractions.
Now that we’ve covered the basics, we can take a closer look at what leucine actually is and the role it plays in your body:
What is leucine?
Leucine is one of the nine essential amino acids and is part of the three branched-chain amino acids (BCAAs). Unlike most other amino acids, BCAAs are mainly broken down in the muscles instead of the liver, which makes them play an important role in repairing and building muscle.
But why is leucine so important?
Leucine is like the “on-switch” for muscle recovery and growth. When leucine levels in your blood reach a certain point, your body gets the signal to kickstart muscle protein synthesis (MPS) — the process that repairs and builds muscle tissue after exercise.
Research shows that around 2.5–3 grams of leucine are needed to fully activate MPS after a workout. If you don’t hit this leucine threshold within 1–2 hours of eating protein, your muscle-building process may only be partially activated — or might not start at all.
What happens if you don’t hit the 2.5-3g leucine threshold?
Leucine signals mTOR, the key pathway for muscle growth (MPS). If you fall short on leucine, mTOR activation is weaker, and your muscles don’t receive the full growth signal. This means that even with enough protein, building muscle is less efficient.
Leucine levels across protein sources
Every protein source has a unique amino acid profile, which means the levels of certain amino acids— like leucine —can vary widely. For example, plant-based proteins such as pea, rice, or hemp are typically lower in leucine, while animal-based proteins like whey are much higher.
This also explains why whey is the world’s most popular post-workout supplement: it has an optimized amino acid profile for supporting muscle recovery and growth.
Whey more powerful
As mentioned earlier, whey is not a single protein. It is actually a blend of several proteins, each with its own unique amino acid profile.
Among these proteins, one clearly stands out due to its significantly higher content of essential amino acids: Beta-lactoglobulin (BLG). Using a patented isolation method, we were able to separate BLG from the other proteins in whey, creating a more pure and potent protein: Pure BLG — the protein used in TWOZERO®.
For comparison: BLG contains 45% more leucine, 26% more essential amino acids and 40% more BCAAs than standard whey (which includes all protein fractions). No wonder we call BLG “whey more powerful”.
Hitting the leucine threshold with BLG
What makes the BLG protein in TWOZERO® such a breakthrough?
It’s simple: it delivers more leucine.
Most protein supplements fail to reach the crucial 2.5–3 gram leucine threshold per serving, but TWOZERO® hits it effortlessly thanks to its BLG protein, which naturally contains far more leucine than standard whey—and even more compared to plant-based proteins like pea.
By the numbers: TWOZERO® delivers 3.2g of leucine per can, while a traditional whey protein shake provides only 2.2 grams and a pea protein shake just 1.7 grams of leucine.
Why is the leucine threshold so important?
Leucine acts as the on-switch for muscle growth. Only when there is enough leucine in your blood — so when the leucine threshold is met — does your body receive the signal to activate muscle protein synthesis (MPS). This is the process where your body repairs and builds muscle.
PART 02
Fractions
How is whey protein composed?
Unlike what many people think, whey protein is not a single protein. It is actually a “soup” of several proteins, known as protein fractions. Each fraction has a unique amino acid profile, meaning the proportions of individual amino acids — both essential and non-essential — vary between fractions.
The key protein fractions in whey
Scientists have discovered that, out of the dozens of protein fractions in whey, 4 key fractions make up nearly 90% of its total protein content. One fraction clearly stands out: Beta-lactoglobulin (BLG), which alone accounts for approximately 55%.
The driving force in whey protein
Beta-lactoglobulin (BLG) is not only the largest protein fraction in whey, but it especially stands out for its extremely high content of essential amino acids, particularly BCAAs. This makes BLG the primary contributor to whey’s muscle-building potential, while the other fractions mainly provide supportive physiological benefits.
Simply put, BLG is the main source of essential amino acids and BCAAs in whey protein. It provides even more than all the other fractions combined.
PART 03
Our innovation
A breakthrough in whey protein
After discovering that a specific protein fraction in whey is responsible for the majority of the amino acids needed for muscle recovery and growth, we asked ourselves: why not isolate this fraction and create a new, more efficient protein — consisting solely of pure BLG?
Years of research and collaboration with top scientists followed, resulting in a breakthrough: using a patented crystallization process, we succeeded in isolating BLG from whey. This innovation resulted in pure BLG protein with three unique functional benefits: efficiency, solubility and taste.
EFFICIENCY
World’s purest and most powerful protein
Our BLG protein is the most powerful form of whey protein available on the market. Not only is it richer in essential amino acids and BCAAs than standard whey, but it is also exceptionally high in leucine — the key amino acid that triggers muscle protein synthesis (MPS).
When comparing BLG to standard whey (which includes all protein fractions), this is how the amino acid profiles differ:
SOLUBILITY
Protein that looks like water
Because of its purity, BLG dissolves far better than regular whey protein. This allows for much higher protein concentrations in drinks — well above the limits of standard whey, where ~14g of protein per 330ml is often the maximum. Thanks to its unique molecular structure, BLG also gives liquids a crystal-clear, water-like appearance.
TASTE
No protein taste or mouthfeel
While a protein drink made with standard whey often has the typical protein taste and dry mouthfeel, this is not the case for a drink made solely from BLG. Thanks to its purity, BLG is the only whey fraction that doesn’t affect taste or texture.
As a result, a protein water made from BLG goes down smoothly — and you’d hardly know it’s protein.The chart below shows the results of a sensory panel study involving 240 athletes. In this study, two protein water drinks were compared — both containing 20g of protein and having identical formulations, but with one key difference: the protein source. One drink contained BLG, while the other consisted of traditional clear whey isolate (which includes all the protein fractions).
Protein comparison
The amino acid composition of Beta-Lactoglobulin compared with other commercially available proteins.
The protein in TWOZERO® clearly outperforms all other protein sources.
Sensory panel study
Taste tested by hybrid athletes.
We conducted a sensory panel study with 240 athletes from a wide range of sports. The TWOZERO® drink, containing pure Beta-Lactoglobulin (BLG), was compared to a protein drink with an identical formulation but with traditional clear whey isolate (WPI) as protein source. Thus, both drinks contained 20g of protein, with the protein type being the only variable.
The study showed a significant difference in how participants experienced the drinks. The TWOZERO® drink was rated as having less typical whey flavor, minimal to no dry mouthfeel and a clearly reduced protein aftertaste compared to the protein water containing traditional whey isolate.
Methodology
Participants
100 male and female athletes, from amateur to elite level, practicing various endurance and strength sports.
Protocol
Assessments were conducted individually, in randomized order, and under controlled conditions. A single-blind design ensured that participants were unaware of the protein source. Water was provided between tastings to neutralize the palate. Sensory attributes were evaluated using a standardized 12-point scale.
Objective
Investigate differences in sensory attributes between protein water drinks containing traditional clear whey protein and those containing pure Beta-Lactoglobulin.
PART 04
The product
From breakthrough to beverage
After discovering the advantages of this innovative protein, we partnered with a renowned flavor house to create a refreshing protein drink that not only performs better but also truly tastes great. The result is a crystal-clear, high-protein beverage with a clean, crisp flavor experience.
No mixing, no shaking, no preparation. Just open the can and enjoy a perfectly balanced protein drink, ready whenever you are.
A short, functional ingredient list. All calories from protein, every ingredient selected for performance and taste.
Lightly carbonated for a crisp, refreshing experience. Clear in texture, smooth to drink, and designed to feel nothing like a traditional shake.
We used advanced protein to craft a drink without the typical protein taste or dry mouthfeel. It’s so refreshing, it might even replace your favorite soda.